Silver chiral nano-flowers with 3-, 4- and 5- fold symmetry were produced using oblique angle deposition method in conjunction with the rotation of sample holder with different speeds at different sectors of each revolution corresponding to symmetry order of the acquired nano-flower. Atomic force microscopy (AFM) and field emission scanning electron microscopy (FESEM), were employed to obtain morphology and nano-structure of the films. Optical characteristics of silver chiral nano-flower thin films were obtained using single beam spectrophotometer with both s- and p-polarization incident light at 30 degrees and 70 degrees incidence angles and at different azimuthal angles (phi). Optical spectra showed both TM (TDM (transverse dipole mode) and TQM (transverse quadruple mode)) and LM (longitudinal mode) Plasmon resonance peaks. For 3- and 4- fold symmetry chiral nano-flowers the s- polarization extinction spectra obtained at different azimuthal angles did not show significant change in the Plasmon peak position while 5- fold symmetry chiral nano-flower showed a completely different behavior, which may be the result of increased surface anisotropy, so when the. angle is changed the s- polarization response from the surface can change more significantly than that for lower symmetries. In general, for 3-, 4- and 5- fold symmetry chiral nano-flowers a sharp peak at lower wavelengths (< 450 nm) is observed in the s- polarization spectra, while in addition to this peak a broad peak at longer wavelengths (i.e., LM) observed in the p-polarization spectra, which is more dominant for 70 degrees incidence angle. (C) 2011 Elsevier B.V. All rights reserved.