We report on the use of zeolites to limit the effects of reactive oxygen species (ROS) on human albumin under in vitro conditions. Zeolites of different structure type, channel size, channel polarity, and charge-compensating cation were screened for the elimination of ROS, notably HO center dot, resulting from the Fenton reaction. A test based on ischemia-modified albumin (IMA) was used as a marker to monitor the activity of HO. after co-exposure of human serum to these zeolites. Two commercial zeolites, faujasite (FAU 13x, channel opening 0.74 x 0.74 nm with Na+ as charge-compensating cation) and ferrierite (FER, channel opening 0.54 x 0.42 nm with H+ as charge-compensating cation), were found to reduce IMA formation by more than 65% due to removal of HO. relative to reference values. It was established that partial ion exchange of the zeolites' respective charge-compensating cation vs. Fe3+ implicated in the Fenton reaction plays a major role in HO. deactivation process. Moreover, our results show that no saturation of the respective zeolite active sites occurred. This is possible only when ROS are actively converted to water molecules within the zeolite void system, which generates H+ ion transport. Because zeolites cannot be administered in blood, their use in medicine should be limited to extra corporeal circuits. Zeolites could be of use during cardiopulmonary bypass or hemodialysis procedures. (C) 2011 Elsevier Inc. All rights reserved.