A rapid and sensitive chemiluminescence (CL) method was designed for the determination of trace amount of perphenazine (PERN) based on the gold nanoparticles enhanced luminol- H2O2-PERN system. The degree of enhancement of CL signal intensity of the system was proportional with the concentration of PERN. Under optimum experimental conditions, the linearity of CL signal intensity was observed in the range of 3.5× 10^(-9) to 2.0× 10^(-7)mol/L (r2= 0.9995) with a detection limit of 1.37× 10^(-9) mol/L. Precision of the method was tested at the concentration level of 1.0× 10^(-7) mol/L for 8 replicate measurements giving value of RSD 2.25%.