Mechanical Characterization of Polysaccharide/Polyaminoacid Hydrogels as Potential Scaffolds for Tissue Regeneration

Pietro Matricardi; Giovanna Pitarresi; Fabio Salvatore Palumbo; Chiara Di Meo; Antonella Albanese; Tommasina Coviello; Claudia Cencetti; Calogero Fiorica; Gaetano Giammona

Macromolecular Research, Vol.19, No.12, 1264-1271, December, 2011

DOI10.1007/s13233-011-1208-y Export Citation

Mechanical Characterization of Polysaccharide/Polyaminoacid Hydrogels as Potential Scaffolds for Tissue Regeneration

Pietro Matricardi¹, Giovanna Pitarresi^{2, 3, †}, Fabio Salvatore Palumbo^{2, 4}, Chiara Di Meo¹, Antonella Albanese^{2, 4}, Tommasina Coviello¹, Claudia Cencetti¹, Calogero Fiorica^{2, 4}, and Gaetano Giammona^{2, 5}

¹Dipartimento di Chimica e Tecnologie del Farmaco, “Sapienza” Universita, Piazzale Aldo Moro 5, 00185, Roma, Italy
²Dipartimento di Scienze e Tecnologie Molecolari e Biomolecolari, Sezione di Chimica e Tecnologie Farmaceutiche, Universita degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy, Korea
³IBIM-CNR, Via Ugo La Malfa 153, 90146, Palermo, Italy
⁴, Korea
⁵Institute of Biophysics at Palermo, Italian National Research Council, Via Ugo La Malfa 153, 90146, Palermo, Italy

E-mail:

The mechanical properties of hydrogel scaffolds based on hyaluronic acid (HA) that were chemically crosslinked with α,β-poly(N-2-hydroxyethyl)(2-aminoethylcarbamate)-D,L-aspartamide (PHEA-EDA) were investigated. Variation of these properties as a function of three different PHEA-EDA amounts used to crosslink HA has been related to the reaction efficiency evaluated using a colorimetric assay. Moreover, the amount of unreacted amino groups that was still present in the hydrogels was related to the attachment behavior of human dermal fibroblasts to the hydrogel surface. The mechanical data and biological results suggest the suitability of the investigated hydrogels as scaffolds for the regeneration of soft tissues such as skin.

Keywords:hyaluronic acid;polyaspartamide;scaffolds;mechanical properties;fibroblast attachment.

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[1] Fraser JR, Laurent TC, Laurent UBG, J. Intern.Med., 242, 27 (1997)

[2] Entwistle J, Hall CL, Turley EA, J. Cell Biochem., 61, 569 (1996)

[3] Kuo JW, Practical Aspects of Hyaluronan-Based Medical Products, CRC Press, New York (2000)

[4] Oh EJ, Park K, Kim KS, Kim J, Yang J, Kong J, Lee MY, Hoffman AS, Hahn SK, J. Control. Release., 141, 2 (2010)

[5] Hahn MS, Teply BA, Stevens MM, Zeitels SM, Langer R, Biomaterials., 27, 1104 (2006)

[6] Shu XZ, Liu Y, Palumbo F, Prestwich GD, Biomaterials., 24, 3825 (2003)

[7] Sahoo S, Chung C, Khetan S, Burdick JA, Biomacromolecules, 9(4), 1088 (2008)

[8] Aguzzi A, Montrasio F, Kaeser PS, Nat. Rev. Mol. Cell Biol., 2, 118 (2001)

[9] Zohuriaan-Mehra MJ, Pourjavadib A, Salimib H, Kurdtabarb M, Polym. Adv. Technol., 20, 655 (2009)

[10] Pitarresi G, Palumbo FS, Cavallaro G, Fare S, Giammona G, J. Biomed. Mat. Res., 87A, 770 (2008)

[11] Giammona G, Pitarresi G, Palumbo FS, WO Patent 001046 A1 (2006)

[12] Pitarresi G, Palumbo FS, Calabrese R, Craparo EF, Giammona G, J. Biomed. Mat. Res., 84A, 413 (2008)

[13] Cavallaro G, Campisi M, Licciardi M, Ogris M, Giammona G, J. Control. Release., 115, 322 (2006)

[14] Pitarresi G, Calabrese R, Palumbo FS, Licciardi M, Giammona G, Int. J. Pharm., 382, 7 (2009)

[15] Shu XZ, Liu YC, Luo Y, Roberts MC, Prestwich GD, Biomacromolecules, 3(6), 1304 (2002)

[16] Giammona G, Carlisi B, Palazzo S, J. Polym. Sci. Part A: Polym. Chem., 25, 2813 (1987)

[17] Mendichi R, Giammona G, Cavallaro G, Schieroni AG, Polymer, 41(24), 8649 (2000)

[18] Kirker KR, Prestwich GD, J. Polym. Sci. B: Polym. Phys., 42(23), 4344 (2004)

[19] Lapasin R, Pricl S, in Rheology of Industrial Polysaccharides: Theory and Applications, Chapman & Hall, London, 162 (1995)

[20] Kuijpers AJ, Engbers GHM, Feijen J, De Smedt SC, Meyvis TKL, Demeester J, Krijgsveld J, Zaat SAJ, Dankert J, Macromolecules, 32(10), 3325 (1999)

[21] Ralston DR, Layton C, Dalley AJ, Boyce SG, Freedlander E, MacNeil S, Br. J. Plast. Surg., 50, 408 (1997)

[22] Clark AH, Ross-Murphy SB, Adv. Polym. Sci., 83, 57 (1987)

[23] Lefebvre J, Doublier JL, in Polysaccharides, S. Dumitriu, Ed., Marcel Dekker Inc., New York, 357 (2005)

[24] Rehfeldt F, Engler AJ, Eckhardt A, Ahmed F, Discher DE, Adv. Drug Deliv. Rev., 59, 1329 (2007)

[25] Freiera T, Montenegroc R, Koha HS, Shoichet MS, Biomaterials., 26, 4624 (2005)

[26] Freiera T, Koha HS, Kazaziana K, Shoichet MS, Biomaterials., 26, 5872 (2005)

[27] Chatelet C, Damour O, Domard A, Biomaterials., 22, 261 (2001)

[28] Pokharna HK, Zhong Y, Smith DJ, Dunphy MJ, J.Bioact. Compat. Polym., 5, 42 (1990)