Mathematical analysis of colonial formation of embryonic stem cells in microfluidic system

Seul Ki Min; Byung Man Lee; Jin Ha Hwang; Sung Ho Ha; Hwa Sung Shin

Korean Journal of Chemical Engineering, Vol.29, No.3, 392-395, March, 2012

DOI10.1007/s11814-011-0181-7 Export Citation

Mathematical analysis of colonial formation of embryonic stem cells in microfluidic system

Seul Ki Min, Byung Man Lee, Jin Ha Hwang, Sung Ho Ha¹, and Hwa Sung Shin^†

Department of Biological Engineering, Inha University, Incheon 402-751, Korea
¹Departmet of Chemical Engineering & Nano-Bio Technology, Hannam University, Daejeon 305-811, Korea

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A fluidic environment affects mechanochemical characteristics of embryonic stem cells (ESCs). Perfusion is recognized as an attractive culture mode of ESCs since the steady fluidic state can enhance ESCs’ controllability, supporting a unique cell culture condition. Cellular membrane motility presents important information about cellular dynamics such as adhesion, spreading, and migration. Thus, an investigation of the perfusion-induced membrane motility is significant to understand the mechanochemical behavior of ESCs in the steady culture state. In this research, we suggest Lfr, the ratio of circumferential membrane unattached to other cells’ to the cell’s circumference, as a new parameter to characterize cells’ shape and motility. Lfr of embryonic stem cells has positive correlations with cellular area (Ar) and free peripheral length (Lf) but a negative correlation with roundness (Rn). We also propose a mathematical model representing ESCs’ membrane motilities and demonstrate their colonical behavior.

Keywords:Embryonic Stem Cells;Morphology;Self-renewal;Perfusion Culture;Free Peripheral Length Ratio (Lfr)

[References]

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[Referenced By]

[1] Odorico JS, Kaufman DS, Thomson JA, Stem Cells., 19, 193 (2001)

[2] O’Brien T, Barry FP, Mayo, Clin. Proc., 84, 859 (2009)

[3] Fong WJ, Tan HL, Choo A, Oh SKW, Bioproc. Biosyst.Eng., 27, 1615 (2005)

[4] Ingber DE, Biol. Bull., 194, 323 (1998)

[5] Tsuboi H, Ando J, Korenaga R, Takada Y, Kamiya A, Biochem.Biophys. Res. Commun., 206, 988 (1995)

[6] Topper JN, Cai J, Falb D, Gimbrone MA, Proc. Natl. Acad.Sci. USA., 93, 10417 (1996)

[7] Hsieh HJ, Li NQ, Frangos JA, Am. J. Physiol., 260, H642 (1991)

[8] Rivilis I, Milkiewicz M, Boyd P, Goldstein J, Brown MD, Egginton S, Hansen FM, Hudlicka O, Haas TL, Am. J. Physiol. Heart Circ. Physiol., 283, H1430 (2002)

[9] Nonaka S, Shiratori H, Saijoh Y, Hamada H, Nature., 418, 96 (2002)

[10] Kimiko Y, Takaaki S, Tetsuro W, Kohei M, Jun KY, Syotaro O, Norihiko O, Akiko M, Akira K, Joji A, Am. J. Physiol. Heart Circ. Physiol., 288, H1915 (2005)

[11] Xia Y, Whitesides GM, Annu. Rev. Mater. Sci., 28, 153 (1998)

[12] Taylor AM, Rhee SW, Tu CH, Cribbs DH, Cotman CW, Jeon NL, Langmuir, 19(5), 1551 (2003)

[13] Shin HS, Kim HJ, Min SK, Kim SH, Lee BM, Jeon NL, Biotechnol. Lett., 32(8), 1063 (2010)

[14] Kim MH, Kino-Oka M, Kawase M, Yagi K, Taya M, J. Biosci. Bioeng., 103(2), 192 (2007)