This paper intends to study the single phase axial dispersion in pulsed sieve plate column using a combination of computational fluid dynamics (CFD) simulations and experimental measurements. Experiments and CFD simulations were conducted on 0.076 m diameter pilot scale column having standard geometry of 0.05 m plate spacing, 0.003 m hole diameter and 0.21 fractional free area. The effect of density of tracer solution and radial probe position on axial dispersion coefficient has been studied to ensure precision of the experimental measurement method. The effect of pulse velocity from 0.01 to 0.025 m/s and superficial velocity of water from 0.01 to 0.03 m/s has been studied. Simulations were carried out using commercial CFD software, FLUENT 6.2.16, with standard k-epsilon model for turbulence. An unsteady state tracer injection technique was used for axial dispersion measurement. The range of velocity ratio (psi = Re-o/Re-n) employed in this work was 1-4 which is very low. Therefore the effect of superficial velocity, V, was found to be greater than pulse velocity. These results were critically compared with published data and it has been found that single phase axial dispersion coefficient is directly proportional to effective velocity (Af + 0.5 V-c). The presented CFD predictions and validation with experimental data will provide useful basis for further work on single phase axial dispersion with various geometrical parameters and understanding the two phase flow patterns in pulsed sieve plate column. (C) 2011 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.