Amine-functionalized mesoporous silica was prepared by using lauric acid and N-stearoyl-1-glutamic acid as structure directing agents via the S-N+-I- mechanism and applied to CO2 adsorption at room temperature. With gamma-aminopropyltriethoxysilane as co-structure directing agent and due to the direct electrostatic interaction with anionic surfactant, most of the amino groups were uniformly distributed at the inner surface of pores and the performance was stable. The amine-functionalized mesoporous silica was characterized by Fourier transform infrared spectrometer, X-ray diffraction, nitrogen physisorption and thermogravimetric analysis. The CO2 adsorption capacity was measured by digital recording balance. At the room temperature and under the atmospheric pressure, the adsorption capacity of LAA-AMS-0.2 for CO2 and N-2 is 1.40 mmol.g(-1) and 0.03 mmol-g(-1), respectively, indicating high separation coefficient of CO2/N-2.