A novel process for preparing pure chenodeoxycholic acid from poultry bile

Junfen Wan; Jiemin He; Xuejun Cao

Journal of Industrial and Engineering Chemistry, Vol.18, No.1, 65-71, January, 2012

DOI10.1016/j.jiec.2011.11.091 Export Citation

A novel process for preparing pure chenodeoxycholic acid from poultry bile

Junfen Wan, Jiemin He, and Xuejun Cao^†

State Key Laboratory of Bioreactor Engineering, Department of Biochemical Engineering, East China University of Science & Technology, Shanghai 200237, China

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Macroporous adsorption by column chromatography was introduced into chenodeoxycholic acid (CDCA) separation from duck bile in this study. After bile saponification in 2.5 M NaOH for 6 h and conversion of CDCA calcium salt with Na2CO3, CDCA was purified by the hydrophobic chromatography with macroporous resin HZ-802. With 40% ethanol as loading solvent and 50% and 60% ethanol as stage elution solvents, high purity of CDCA was obtained, and its melting point could be achieved 160-170 ℃ (99% purity) after crystallization by 65-70 ℃ water bath cultivation. The novel process was environmentally friendly and economical compared to conventional method, and it has been successfully scaled up in industry.

Keywords:Chenodeoxycholic acid;Bile acids;Adsorption;Chromatography;Crystallization

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