In this work, we analyzed the color and texture of irises, ocular prostheses, and cosmetic colored contact lenses measured by means of a multispectral system, which provides the CIE L*a*b* colorimetric coordinates of a high resolution image pixel by pixel. The same subject, who has dark brown irises, participated in the measurement of all the contact lenses. The CIE L*a*b* colorimetric coordinates were analyzed to classify the samples into three major groups (brown, blue and green) using a new algorithm developed for this purpose. This classification allowed us to carry out a comparison of the color associated with each set of samples, using the corresponding color gamuts in the CIE L*a*b* color space. Furthermore, we analyzed the iris color reproduction achieved by prostheses and contact lenses in terms of CIEDE2000 color differences, and obtained closer results with prostheses. In addition, we performed an analysis of texture by means of the color spatial distribution of all samples. This was achieved by means of two statistical approaches: first order statistics of image histograms and second order statistics using co-occurrence matrices. The results suggest that the texture associated with real irises, ocular prostheses and colored contact lenses is very different. This study provides useful information about the color and texture of irises that may help to establish a strategy for improving the techniques used in the manufacturing process of prostheses and colored contact lenses to obtain a better and more realistic appearance. (C) 2010 Wiley Periodicals, Inc. Col Res Appl, 36, 373-382, 2011; Published online 7 October 2010 in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/col.20635