Nanoporous gold (NPG) fabricated by dealloying Au-Ag film was investigated for the non-enzymatic detection of H2O2. The apparent activation energy of H2O2 electrochemical reduction on NPG was found to be as low as similar to 30 kJ mol(-1). The reduction currents at -0.4V vs. SCE demonstrated a strict linear dependence in a wide H2O2 concentration region from 10 mu M to 8 mM with a detection limit 3.26 mu M. Furthermore, the biosensor based on NPG exhibited high selectivity, good reproducibility, and long-term stability. These results indicate that NPG could be a promising electrochemical material for H2O2 detection. (C) 2011 Elsevier Ltd. All rights reserved.