Electrochemical (EC) quantification of 11-Mercaptoundecylferrocene (thiolated-ferrocene substrate, Fc-SH) onto gold nanoparticles (AuNPs) was carried out through cyclic voltammetric (CV) investigations in different base electrolyte aqueous media on glassy carbon (GC) electrode. The electrochemical data including peak current, peak potential values revealed the Fc-SH-AuNPs redox system to be adsorption controlled in terms of self assembled monolayers (SAMs). The electrochemically generated SAMs were found quite stable as these maintained the redox activity upto 3 months after repeated CV scans at 298 K. The overall output of this research can be utilized in two ways: application of electroactivity and stability of these SAMs in detection of biologically important molecules and also towards the development of EC biosensors. (C) 2011 Elsevier Ltd. All rights reserved.