In Part 1 of this work, a theoretical description of gradient elution moving boundary electrophoresis (GEMBE) is presented along with a comparison between GEMBE and the more conventional technique of CZE. In this second part, experimental results are presented for comparison with the theoretical predictions of Part 1. Results for the step width, spacing, and resolution are examined as a function of the salient parameters affecting the results of a GEMBE separation (starting pressure, counterflow acceleration, and electric field strength).