화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.36, No.2, 124-130, March, 2012
Export Citation
전기 방사법을 통해 제조된 Poly(L-lactide-co-ε -caprolactone)/Marine Collagen 나노파이버의 특성 및 세포친화력 평가
Characteristics and Biocompatibility of Electrospun Nanofibers with Poly(L-lactide-co-ε-caprolactone)/Marine Collagen
김우진1, 2, 신영민1, 박종석1, 권희정1, 김용수1, 2, 신흥수2, 노영창1, 임윤묵1, †, 정무상3
  • 1한국원자력연구원 정읍방사선과학연구소 방사선공업환경연구부
  • 2한양대학교 응용화학생명공학부 생명공학과
  • 3제주한라대학교 임상병리과
Woo-Jin Kim1, 2, Young Min Shin1, Jong-Seok Park1, Hui-Jeong Gwon1, Yong-Soo Kim1, 2, Heungsoo Shin2, Young-Chang Nho1, Youn-Mook Lim1, †, and Moo Sang Chong3
  • 1Radiation Research Division for Industry and Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 1266 Sinjeong-dong, Jeongeup-si, Jeollabuk-do 580-185, Korea
  • 2Department of Bioengineering, Division of Applied Chemical and Bio Engineering, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, Korea
  • 3Department of Clinical Pathology, Cheju Halla University, Jeju Special Self-Governing Province 690-708, Korea
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초록
본 연구의 목적은 전기방사법을 사용하여 poly(L-lactide-co-ε-caprolactone) (PLCL)과 marine collagen (MC)이 혼합된 나노섬유를 제조하는 것이다. 전기방사된 나노섬유의 직경과 형태는 여러 공정 변수에 의해서 변화되는데, PLCL과 MC의 혼합비, 노즐과 콜렉터와의 거리, 노즐의 직경, 용액의 방출 속도 그리고 전기장의 세기 변화에 따라 나노파이버의 직경을 주사전자현미경을 통해서 분석하였다. 또한 제조된 나노파이버의 표면변화를 확인하기 위해 물과의 접촉각을 측정하였으며, 나노파이버의 세포 친화성을 평가하기 위해 MG-63을 이용하여 생존율과 흡착형태를 주사전자현미경과 형광현미경을 통해서 관찰하였다. 이와 같은 연구 결과, 방사거리, MC의 함량, 전기장의 세기가 증가할수록 제조된 나노파이버의 평균직경은 감소하는 경향을 나타냈다. 또한 MC의 함량이 증가할수록 나노파이버의 친수성이 증가하였고 세포독성은 관찰되지 않았다. 이에 따라 해양유래 생물에서 추출한 콜라겐은 조직공학용 소재에 새롭게 사용될 수 있을 것으로 예상된다.
The uniform nanofibers of poly(L-lactide-co-ε-caprolactone) (PLCL) with different contents of marine collagen (MC) were successfully prepared by electrospinning method. The effects of the major parameters in electrospinning process such as tip to target distance (TTD), voltage, nozzle size and flow rate on the average diameter of the electrospun nanofiber were investigated in generating composite nanofiber. The diameter and morphology of the nanofibers were confirmed by a scanning electron microscopy (SEM). Also, we measured a water contact angle to determine the surface wettability of the nanofibers. The average diameter of the nanofibers decreased as the value of TTD, MC contents, and voltages increased in comparison with that of pristine PLCL nanofiber. In contrast, the diameter of the nanofibers increased as the flow rate and inner diameter of nozzle increased in comparison with that of pristine PLCL. In addition, the hydrophilicity of the nanofiber and attachment of MG-63 cells on the sheets increased as incorporated collagen contents increased. Therefore, the marine collagen would be a potential material to enhance cellular interactivity of synthetic materials by mimicking the natural tissue.
Keywords:poly(L-lactide-co-ε-caprolactone);marine collagen;nanofiber;electrospinning.
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