다중에멀젼법을 통한 슈도모나스를 함유하는 PCL 마이크로캡슐의 제조 및 특성 연구

김기석; 이승엽; 이건웅; 김형곤; 박수진; Ki-Seok Kim; Seung Yeop Lee; Gun Woong Lee; Hyung Gon Kim; Soo-Jin Park

Polymer(Korea), Vol.36, No.2, 202-207, March, 2012

Export Citation

다중에멀젼법을 통한 슈도모나스를 함유하는 PCL 마이크로캡슐의 제조 및 특성 연구

Preparation and Characteristics of Poly(ε-caprolactone) Microcapsules Containing Pseudomonas by W/O/W Emulsion

김기석 ¹, 이승엽², 이건웅^{3, 4}, 김형곤², 박수진 ^{1, †}

¹인하대학교 화학과
²(주)캠스텍
³형재바이오
⁴전북대학교 생명공학부

Ki-Seok Kim¹, Seung Yeop Lee², Gun Woong Lee^{3, 4}, Hyung Gon Kim², and Soo-Jin Park^{1, †}

¹Department of Chemistry, Inha University, Incheon 402-751, Korea
²Camstech, 405, JMC, 762, Palbokdong 2Ga, Duckjin-gu, Jeonju 561-844, Korea
³Hyung Jae Bio, Business incubator Center, Chonbuk National University, Jeonju 561-756, Korea
⁴Division of Biotechnology, Chonbuk National University, Iksan 570-752, Korea

E-mail:

초록

슈도모나스를 함유하는 poly(ε-caprolactone)(PCL) 마이크로캡슐은 다중에멀젼 시스템에 의하여 제조하였고, 제조조건에 따른 마이크로캡슐의 특성과 방출거동에 대하여 조사하였다. 마이크로캡슐의 형태와 입도분포는 주사전자현미경과 입도분석기를 이용하여 관찰하였고, 방출거동은 액체배양법을 통하여 확인하였다. 다양한 제조조건에 따라 제조된 마이크로캡슐은 10~60 μm의 입자크기와 표면이 매끈하고 균일한 구형의 마이크로캡슐이 형성됨을 확인하였다. 열분석 결과, 마이크로캡슐은 약 58 ℃에서 용융피크를 나타내었고 벽재물질의 함량 증가에 따라 용융열이 증가함으로써 벽재의 두께는 PCL 함량에 비례함을 확인할 수 있었다. 또한 슈도모나스의 방출속도는 유화제 함량, 교반속도, PCL 함량에 따라 조절이 가능하며 이는 유화제 함량과 교반속도 증가에 따른 입자 크기의 감소에 의한 비표면적 증가와 벽재물질의 두께 증가에 인한 방출속도의 감소에 의한 것으로 판단된다.

Biodegradable poly(ε-caprolactone)(PCL) microcapsules containing pseudomonas were prepared by W/O/W emulsion system. The characteristics and release behaviors of the microcapsules were investigated as a function of manufacturing conditions. The morphology and particle distribution of the microcapsules were observed by a scanning electron microscope and a particle size analyzer. The release behaviors of the pseudomonas were determined using a cell culture method. It was found that smooth and spherical microcapsules were formed by W/O/W emulsion system and particle size was in the range of 10 to 60 μm. The release behaviors of the pseudomonas were influenced by the manufacturing conditions. It was indicated that the increase of the surfactant content and stirring rate led to an increased release rate, resulting from the high specific surface area of the smaller particle size, and the increase of the PCL content provided the sustained release behaviors by the delay effect of diffusion in the release medium.

Keywords:microcapsules;poly(ε-caprolactone);pseudomonas;release behavior.

[References]

Scherwinski K, Grosch R, Berg G, FEMS Microbiol.Ecol., 64, 106 (2008)
Cha MS, Lim EG, Lee KH, Cho SJ, Son HJ, Lee SJ, J. Environ. Sci., 11, 177 (2002)
Karamalidis AK, Evangelou AC, Karabika E, Koukkou AI, Drainas C, Voudrias EA, Bioresource Technol., 101, 6545 (2010)
Van Dyke MI, Prosser JI, Soil Biology Biochem., 32, 1377 (2000)
Stockwell VO, Stack JP, Phytopathology., 97, 244 (2007)
Weller DM, Phytopathology., 97, 250 (2007)
Svercel M, Hamelin J, Duffy B, Moenne-Loccoz Y, Defago G, Soil Biology Biochem., 42, 466 (2010)
Johansson PM, Wright SAI, Appl. Environ. Microbiol., 69, 6464 (2003)
Mathre DE, Cook RJ, Callan NW, Phytopathology., 83, 972 (1999)
Park SJ, Kim KS, Kim SH, Colloids Surf. B., 43, 238 (2005)
Park JM, Park SJ, Macromol. Res., 18(12), 1191 (2010)
Benoit JP, Marchais H, Rolland H, Velde VV, Microencapsulation Methods and Industrial Applications, Simon Benita, Marcel Dekker, New York (1996)
Erdem B, Sudol ED, Dimonie VL, El-Aasser MS, J. Polym. Sci. A: Polym. Chem., 38(24), 4441 (2000)
Kooiman K, Bohmer MR, Emmer M, Vos HJ, Chlon C, Shi WT, Hall CS, de Winter SHP, Schroen K, Versluis M, de Jong N, van Wamel A, J. Control. Release., 133, 109 (2009)
Loxley A, Vincent B, J. Colloid Interface Sci., 208(1), 49 (1998)
Kim KS, Park SJ, Colloids Surf. B., 76, 404 (2010)
Yilmaz G, Jongboom ROJ, Feil H, Hennink WE, Carbohydr. Polym., 45, 404 (2001)
Kim A, Park SJ, Lee JR, J. Colloid Interface Sci., 197(1), 119 (1998)
Valot P, Baba M, Nedelec JM, Sintes-Zydowicz N, Int. J. Pharm., 369, 53 (2009)
Arriagada FJ, Osseo-Asare K, J. Colloid Interface Sci., 211(2), 210 (1999)
Kilicarslan M, Baykara T, Int. J. Pharm., 252, 99 (2003)
O’Donnell PB, McGinity JW, Adv. Drug Deliever. Rev., 28, 25 (1997)
Rafati H, Coombes AGA, Adler J, Holland J, Davis SS, J. Control. Release., 43, 89 (1997)
Park SJ, Kim KS, Colloids Surf. B., 43, 138 (2005)
Bachtsi A, Kiparissides C, J. Control. Release., 38, 49 (1996)
Hunter JR, Foundations of Colloid Science, Clarendon Press, Oxford (1987)

[Referenced By]

[Related Articles]

[1] Scherwinski K, Grosch R, Berg G, FEMS Microbiol.Ecol., 64, 106 (2008)

[2] Cha MS, Lim EG, Lee KH, Cho SJ, Son HJ, Lee SJ, J. Environ. Sci., 11, 177 (2002)

[3] Karamalidis AK, Evangelou AC, Karabika E, Koukkou AI, Drainas C, Voudrias EA, Bioresource Technol., 101, 6545 (2010)

[4] Van Dyke MI, Prosser JI, Soil Biology Biochem., 32, 1377 (2000)

[5] Stockwell VO, Stack JP, Phytopathology., 97, 244 (2007)

[6] Weller DM, Phytopathology., 97, 250 (2007)

[7] Svercel M, Hamelin J, Duffy B, Moenne-Loccoz Y, Defago G, Soil Biology Biochem., 42, 466 (2010)

[8] Johansson PM, Wright SAI, Appl. Environ. Microbiol., 69, 6464 (2003)

[9] Mathre DE, Cook RJ, Callan NW, Phytopathology., 83, 972 (1999)

[10] Park SJ, Kim KS, Kim SH, Colloids Surf. B., 43, 238 (2005)

[11] Park JM, Park SJ, Macromol. Res., 18(12), 1191 (2010)

[12] Benoit JP, Marchais H, Rolland H, Velde VV, Microencapsulation Methods and Industrial Applications, Simon Benita, Marcel Dekker, New York (1996)

[13] Erdem B, Sudol ED, Dimonie VL, El-Aasser MS, J. Polym. Sci. A: Polym. Chem., 38(24), 4441 (2000)

[14] Kooiman K, Bohmer MR, Emmer M, Vos HJ, Chlon C, Shi WT, Hall CS, de Winter SHP, Schroen K, Versluis M, de Jong N, van Wamel A, J. Control. Release., 133, 109 (2009)

[15] Loxley A, Vincent B, J. Colloid Interface Sci., 208(1), 49 (1998)

[16] Kim KS, Park SJ, Colloids Surf. B., 76, 404 (2010)

[17] Yilmaz G, Jongboom ROJ, Feil H, Hennink WE, Carbohydr. Polym., 45, 404 (2001)

[18] Kim A, Park SJ, Lee JR, J. Colloid Interface Sci., 197(1), 119 (1998)

[19] Valot P, Baba M, Nedelec JM, Sintes-Zydowicz N, Int. J. Pharm., 369, 53 (2009)

[20] Arriagada FJ, Osseo-Asare K, J. Colloid Interface Sci., 211(2), 210 (1999)

[21] Kilicarslan M, Baykara T, Int. J. Pharm., 252, 99 (2003)

[22] O’Donnell PB, McGinity JW, Adv. Drug Deliever. Rev., 28, 25 (1997)

[23] Rafati H, Coombes AGA, Adler J, Holland J, Davis SS, J. Control. Release., 43, 89 (1997)

[24] Park SJ, Kim KS, Colloids Surf. B., 43, 138 (2005)

[25] Bachtsi A, Kiparissides C, J. Control. Release., 38, 49 (1996)

[26] Hunter JR, Foundations of Colloid Science, Clarendon Press, Oxford (1987)