Extraction of phytic acid from HCl extract of rapeseed meal using Alamine 336/n-octanol/sulfonated kerosene was investigated. The results showed that the addition of n-octanol can significantly enhance the extraction of phytic acid by Alamine 336/sulfonated kerosene, indicating the synergetic effect between Alamine 336 and n-octanol. The key affecting factors of the extraction include pH and the concentration of the extractants, whose effects on the distribution ratio were quantitatively analyzed. Phytic acid extracted into the organic solutions can be well strip extracted back by using a dilute NaOH solution. At the optimum operating conditions, the extraction efficiency from a 0.03 mol/L phytic acid solution can reach 85.4%, and the corresponding single strip-extraction efficiency is 74.6%, while the total strip-extraction efficiency after three strip extractions can reach 96.4%.