Industrial & Engineering Chemistry Research, Vol.51, No.1, 255-261, 2012
Sol-Gel Derived Boehmite as an Efficient and Robust Carrier for Enzyme Encapsulation
Boehmite particles were prepared by a facile sol-gel method using sodium aluminate as the precursor under ambient conditions, and they were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), powder X-ray diffraction (XRD), nitrogen adsorption, etc. It is found that the boehmite particles consist of flat nanostrips, which are about 230 nm in length and 30 nm in width. The nanostrips are composed of nanoneedles of about 7 nm in diameter and 24 nm in length. The Brunauer-Emmett-Teller (BET) specific surface area, pore size, and pore volume of boehmite particles are 165 m(2) g(-1), 3.8 nm, and 0.11 cm(3) g(-1), respectively. Moreover, these boehmite particles are used as an efficient and robust carrier to encapsulate a model enzyme: bovine liver catalase. The catalase encapsulated in boehmite particles exhibits higher pH, thermal, and storage stabilities compared to its free counterpart. These results indicate that boehmite may become a competitive carrier for biological and technological applications.