We proposed a systematic strategy based on the studies about the adsorption performance of AB-8 macroporous adsorption resins for prediction of the equilibrium time in taxifolin isolation. Batch experiments were appropriately designed to obtain the best fitting model and to gain insight into the adsorption mechanisms. A modified Langmuir model was used to calculate the adsorption capacity at the adsorption equilibrium point (Q(e)). Subsequently, the reformed pseudo-second-order model was employed to predict the effects of the second-order rate index (k(2)Q(e)) on the adsorption time (t). Then, the effects of the initial adsorption factor (R-i) on the relative adsorption time (t/t(e)) were predicted by the reformed intraparticle diffusion model. On the basis of mathematical deduction, the value of the adsorption equilibrium time (t(e)) can be predicted, which may lead to reduced sampling in adsorption kinetics studies. Furthermore, the good agreement between experimental and predicted data confirmed the reliability of the proposed strategy.