A simple and sensitive fluorometric method has been proposed by the enhanced luminescence from the europium (Eu3+)-doxycycline (DC) complex in the presence of silver nanoparticles (AgNPs). The enhancement of the luminescence from the Eu3+-DC complex by AgNPs was accredited due to the transfer of resonance energy to the fluorophores through the interaction of the excited-state fluorophores and surface plasmon electron in the metal nanosurface. The enhanced luminescence intensity was linearly proportional to the concentration of DC in the range of 3.0×10^(-9)-1.7×10^(-7) g mL^(-1). The limit of detection was found to be 4.0×10^(-10) g mL^(-1). The proposed method has been applied successfully to determine DC in pharmaceuticals and biological samples.