Arbitrary, Complex Cell Patterning via Inkjet Printing of a Cell Membrane-Anchoring Polymer

Hwa In Yoon; Ji Suk Choi; Byung Kook Lee; Je Hoon Oh; Yong Woo Cho

Macromolecular Research, Vol.20, No.5, 528-533, May, 2012

DOI10.1007/s13233-012-0068-4 Export Citation

Arbitrary, Complex Cell Patterning via Inkjet Printing of a Cell Membrane-Anchoring Polymer

Hwa In Yoon, Ji Suk Choi, Byung Kook Lee, Je Hoon Oh¹, and Yong Woo Cho^†

Department of Chemical Engineering and Department of Bionanotechnology, Hanyang University, Gyeonggi, 426-791, Korea
¹Department of Mechanical Engineering, Hanyang University, Gyeonggi, 426-791, Korea

E-mail:

Well-defined cell patterns, including both adherent and non-adherent cells, were created using piezoelectric inkjet printing of a cell membrane-anchoring polymer composed of a hydrophobic oleyl group, a hydrophilic poly(ethylene glycol) (PEG) chain, and an amino-reactive N-hydroxy-succinimide (NHS) end group. Various complex living cell patterns were created by surface engineering in which inkjet-printed, membrane-anchoring polymer patterns controlled the geometric distributions of immobilized cells. Non-adherent (SNU-620) and adherent cells(HeLa) were stably anchored and patterned on a glass substrate through interactions between cell membranes and the hydrophobic oleyl groups. The effects of an apoptosis inducer (staurosporine) and an anticancer drug (paclitaxel) were visualized on cells immobilized on the polymer-printed patterns. This approach may have broad utility in most advanced biomedical devices requiring miniaturized and precisely controlled living cell patterns.

Keywords:cell membrane-anchoring polymer;cell array;cell patterning;drug screening;piezoelectric inkjet printing.

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[9] Dijksman JF, Duineveld PC, Hack MJJ, Pierik A, Rensen J, Rubingh JE, Schram I, Vernhout MM, J.Mater. Chem., 17, 511 (2007)

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