Aging 효과에 따른 나노메탈(Au, Ag)-이산화티탄 복합체의 항균 활성도

박혜림; 이상화; 유인상; Hye-Rim Park; Sang-Wha Lee; In-Sang Yoo

Applied Chemistry for Engineering, Vol.23, No.3, 293-296, June, 2012

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Aging 효과에 따른 나노메탈(Au, Ag)-이산화티탄 복합체의 항균 활성도

Aging Effect on the Antimicrobial Activity of Nanometal (Au, Ag)-Titanium Dioxide Nanocomposites

박혜림, 이상화 ^†, 유인상

가천대학교 화공생명공학과

Hye-Rim Park, Sang-Wha Lee^†, and In-Sang Yoo

Department of Chemical and Biochemical Engineering, Gachen University, Gyeonggi-do 461-701, Korea

E-mail:

초록

항균 활성도를 조사하기 위해 이산화티탄미립자 표면에 나노메탈(Au, Ag)을 균일하게 도핑한 나노복합체(Ag-TiO2, Au-TiO2, Ag-TiO2@TiOx)를 초음파환원법과 졸-겔 법으로 제조하였다. 이렇게 제조된 나노메탈-이산화티탄 복합체의 항균 활성도는 고체배지 표면에 나노복합체와 함께 E. coli를 도말하여 37 ℃에서 배양한 후 생존된 콜로니 개수의 측정을 통해서 이루어졌다. 나노복합체의 초기 항균 효율은 나노메탈이 도핑된 이산화티탄미립자(Ag-TiO2, Au-TiO2, Ag-TiO2@TiOx) 복합체가 도핑하지 않은 이산화티탄미립자(TiO2)에 비해 높은 항균 효율을 나타내주었다. 이후, 나노복합체를 4 ℃에서 보관 후 장기보관에 따른 항균특성을 비교해본 결과, 나노메탈만 도핑된 복합체(Ag-TiO2, Au-TiO2) 보다는 Ag-TiO2의 표면을 다시 산화티탄막으로 코팅한 복합체(Ag-TiO2@TiOx)의 항균특성이 오랫동안 유지되는 것을 알수 있었다. 이는 산화티탄막으로 다시 코팅한 Ag-TiO2@TiOx의 경우에 Ag 나노메탈의 산화방지 및 나노복합체의 콜로이드 안정성 향상이 이루어졌기 때문으로 사료된다.

Nanocomposites were fabricated as titanium dioxide (TiO2) doped with nanometals (Au, Ag) by sonochemical reduction method and sol-gel method in order to investigate their antimicrobial activities. Then, the antimicrobial activity of the resulting samples was compared by the measurement of colony numbers survived on the agar plate incubated for 24 h after the loading E. coli on the solid-state media with the nanocomposites. The initial antimicrobial activity of the metal (Au, Ag)-doped TiO2 was higher than that of the pristine TiO2. Afterwards the nanocomposite samples were kept at 4 ℃ for a long time and the aged samples exhibited the different antimicrobial activity. With the elapse of aging times, Ag-doped TiO2 with TiO2 coating (Ag-TiO2@TiOx) exhibited the higher antimicrobial activity than those of Ag-TiO2 and Au-TiO2. The TiO2 coating on the Ag-TiO2 may prevent the oxidation of Ag nanometals and stabilize colloidal nanocomposites.

Keywords:nanometal;TiO2;Au;Ag;sonochemical deposition

[References]

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Hoffmann MR, Martin ST, Choi WY, Bahnemann DW, Chem. Rev., 95(1), 69 (1995)
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Rai M, Yadav A, Gade A, Biotechnology Advances., 27, 76 (2009)
Lee CJ, Kim DY, Kim BS, J. Korean Ind. Eng. Chem., 18(4), 396 (2007)
Yang D, Park SE, Lee JK, Lee SW, J. Cryst. Growth, 311(3), 508 (2009)
Yang D, Kim S, Lee S, J Nanosci. Nanotech., 11, 6067 (2011)
Joo HK, J. Korean Agric. Chem. Soc., 35, 294 (1992)
AshaRami PV, Mun GLK, Hande MP, Valiyaveettil S, ACS Nano., 3, 279 (2009)
Rai M, Yadav A, Gade A, Biotechnology Advances., 27, 76 (2009)

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[2] Jang HD, Kim SK, Prospect. Ind. Chem., 3(6), 41 (2000)

[3] Hoffmann MR, Martin ST, Choi WY, Bahnemann DW, Chem. Rev., 95(1), 69 (1995)

[4] Oillis DF, Hisio CY, Budiman L, Lee CH, J. Catal., 88, 89 (1984)

[5] Xu M, Bao S, Zhang X, Mater. Lett., 59, 2194 (2005)

[6] Wu XF, Song HY, Yoon JM, Yu YT, Chen YF, Langmuir, 25(11), 6438 (2009)

[7] Yu C, Ho W, Lin J, Yip H, Wang PK, Environ. Sci.Technol., 37, 2296 (2003)

[8] Sunada K, Kikuchi Y, Hashimoto K, Fujishima A, Environ.Sci. Technol., 32, 726 (1998)

[9] Koo G, Jang J, J. of the Korean Soc. of Dyes and Finishers., 20, 1 (2008)

[10] Romanowski P, Schueller R, Beginning Cosmetic Chemistry, ed. Kozlowski AC, 1, 431, Alluredbooks, IL, USA (2009)

[11] Nohyneka GJ, Dufoura EK, Roberts MS, Skin Pharmacol.Physiol., 21, 136 (2008)

[12] AshaRani PV, Mun GLK, Hande MP, Valiyaveettil S, ACS Nano., 3, 279 (2009)

[13] Rai M, Yadav A, Gade A, Biotechnology Advances., 27, 76 (2009)

[14] Lee CJ, Kim DY, Kim BS, J. Korean Ind. Eng. Chem., 18(4), 396 (2007)

[15] Yang D, Park SE, Lee JK, Lee SW, J. Cryst. Growth, 311(3), 508 (2009)

[16] Yang D, Kim S, Lee S, J Nanosci. Nanotech., 11, 6067 (2011)

[17] Joo HK, J. Korean Agric. Chem. Soc., 35, 294 (1992)

[18] AshaRami PV, Mun GLK, Hande MP, Valiyaveettil S, ACS Nano., 3, 279 (2009)

[19] Rai M, Yadav A, Gade A, Biotechnology Advances., 27, 76 (2009)