High-throughput screening of improved protease inhibitors using a yeast cell surface display system and a yeast cell chip

Aoki W; Yoshino Y; Morisaka H; Tsunetomo K; Koyo H; Kamiya S; Kawata N; Kuroda K; Ueda M

Journal of Bioscience and Bioengineering, Vol.111, No.1, 16-18, 2011

DOI10.1016/j.jbiosc.2010.08.006 Export Citation

High-throughput screening of improved protease inhibitors using a yeast cell surface display system and a yeast cell chip

Aoki W, Yoshino Y, Morisaka H, Tsunetomo K, Koyo H, Kamiya S, Kawata N, Kuroda K, Ueda M

Protease-targeted inhibitors have been promising pharmaceuticals. Here, we combined a yeast cell surface display system with a yeast cell chip for the high-throughput screening of protease inhibitors, and succeeded in improving the activity of a protease inhibitor. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.

Keywords:High-throughput screening system;Yeast cell surface display;Yeast cell chip;Automatic single-cell pickup machine;Neurolysin;Protease inhibitor;Matrix metalloprotease