An actinomycete that excretes inulin fructotransferase to the culture supernatant was able to produce di-o-fructofuranose 1,2':2,3' dianhydride (DFA III) from inulin, with the greatest rate of enzyme activity at 65 degrees C and at a pH of 5.5. Through chemotaxonomic and 165 rRNA gene analysis, this strain was identified as genus Nonomuraea in the Streptosporangiaceae family. This is the first report of an inulin fructotransferase producer in this family. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.