When oil is mixed with light alkanes, asphaltenes can precipitate out of oil solutions in a multistep process that involves the formation of nano and colloidal scale particles, the aggregation of asphaltene colloids and their eventual sedimentation Amphiphilic dispersants can greatly affect this process The mechanism of the dispersant action in colloidal asphaltene suspensions in heptane has been shown through previous work to be due in part to a reduction in the size of the colloidal asphaltene particles with the addition of dispersant However, previous studies of the sedimentation behavior revealed evidence of aggregation processes in the colloidal asphaltenes in heptane that has yet to be investigated fully We investigate the effect of dispersants on this aggregation behavior through the use of dynamic light scattering showing that both the amount of dispersant and the amount of heptane dilution can slow the onset of aggregation in colloidal asphaltene suspensions An effective dispersant acts by suppressing colloidal aggregation in asphaltene suspensions as shown by light scattering and therefore also slows separation from the bulk as revealed through macroscopic sedimentation experiments