The effect of monosialoganglioside GM1 (GM1) concentration on the lateral phase separation in the sphingomyelin/1,2-dioleoyl-sn-glycero-3-phosphocholine/cholesterol (SM/DOPC/Chol) bilayers was studied by using atomic force microscopy. The results show that, with the increase of GM1 mol fraction (x), the dominant composition of liquid-ordered (L(o)) domains changes from SM to SM/GM1 and finally to GM1. Meanwhile, the decrease of domain area (A) of the L(o) phase with the increase of x follows a scaling law of A similar to x (-3/2), for x > 0.005, indicating that the domain growth is pinned with high GM1 concentration. Results of in situ experiments of GM1 insertion into SM/DOPC/cholesterol bilayers further supported our observations.