A well-defined triblock terpolymer, poly(ethylene glycol)-block-poly(allyl glycidyl ether)-block-poly(tert-butyl glycidyl ether) (PEG-b-PAGE-b-Pt-BGE), with a narrow molar mass distribution has been synthesized by sequential living anionic ring-opening polymerization. Afterward, the PAGE block was modified via thiol-ene chemistry and different sugar moieties or cysteine as a model compound for peptides could be covalently attached to the polymer backbone. The solution self-assembly of the obtained bis-hydrophilic triblock terpolymers in aqueous media has been studied in detail by turbidimetry, dynamic light scattering, and transmission electron microscopy (TEM and cryo-TEM). (C) 2012 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2012