Journal of the American Chemical Society, Vol.133, No.22, 8474-8477, 2011
Rapid Detection of a Cocaine-Binding Aptamer Using Biological Nanopores on a Chip
This paper describes a methodology for the rapid and highly selective detection of cocaine using a membrane protein channel combined with a DNA aptamer. The DNA aptamer recognizes the cocaine molecule with high selectivity. We successfully detected a low concentration of cocaine (300 ng/mL, the drug test cutoff limit) within 60 s using a biological nanopore embedded in a microchip.