Floating monolayer mixtures of cationic dioctadecyldimethyldiammonium bromide and anionic lipids were used as variable templates for the biomimetic nucleation of calcium carbonate and studied using grazing incidence X-ray diffraction. Varying the ratio of constituents changes the monolayer charge, structure, and molecular tilt. The nucleating surface of calcite also changes as the mixture is varied, and at an 80:20 ratio the (012) face is seen under a floating monolayer template for the first time. Our results indicate that the average template lattice is the major controlling factor in the oriented nucleation of CaCO(3). This is in contrast to the current view that the orientation is controlled by the stereochemical matching of the terminal functional group and molecular tilt with respect to the carbonate groups in the crystal.