Novel amphiphilic ethyl cellulose (EC) brush polymers with mono and dual side chains of poly(2-(2-methoxyethoxy)ethyl methacrylate)-co-oligo(ethylene glycol) methacrylate) (P(MEO(2)MA-co-OEGMA)) and poly(2-(N,N-dimethylamino)ethyl methacrylate) (PDMAEMA) were synthesized by the combination of atom transfer radical polymerization (ATRP) and click chemistry. The molar ratio of P(MEO(2)MA-co-OEGMA) and PDMAEMA was varied through changing the feed ratio of these polymers and the coupling efficiency of click chemistry is relatively high. The brush polymers can self-assemble into spherical micelles/aggregates. The micelles/aggregates show the tunable temperature-pH responsive properties. The cloud points and the pH-triggered phase transition were influenced by EC chains and the ratio of P(MEO(2)MA-co-OEGMA) and PDMAEMA side chains. The brush polymers have the great potential applications as biomedical or intelligent materials. (C) 2012 Elsevier Ltd. All rights reserved.