In the latest two decades, the interest received by plant enzymes has increased significantly. Plant enzymes such as peroxidases are widely used in medicine as diagnostic tools and in the bioremediation and biobleaching industries, among others. Traditionally, these enzymes have been obtained from a natural source, a process that is sometimes laborious and affected by weather conditions and low yields. To overcome this hurdle, some efforts have been made to establish plant cell cultures in vitro to use the system as a continuous source of plant enzymes. The focus of this review will be the production of plant peroxidases in vitro, including novel approaches such as the use of bioreactors and genetically transformed tissues to enhance the yield of desired enzymes.