A complete conversion of lignocellulosic biomass requires different steps, which may interact and interfere during a process cascade. This work focuses on sustainable applications using exclusively water, carbon dioxide and enzymes. A comparison of the relevant process parameters pressure, temperature and residence time shows that these different approaches may be combined in a one-step unit. By this method an objective and efficient screening of a large frame of biomass residuals is possible. Additionally, the interactions between the biomass compounds have been studied, and a reasonable cascade of processes has been deduced, which minimizes the generation of side products and leads to high value products.