For this study, ten complex class 1 integron was characterized in Escherichia coli (E. coli) isolates from Zhenjiang. Among the ten isolates, five included the bla(CTX-M-1) gene between orf513 and the IS 3000. Analysis the five isolates by PFGE showed that zj102 and zj562 had same patterns profiles, which suggested that the spread of a specific clone contributed to a wide dissemination of a particular type of integron; the results also indicated that the complex class 1 integron might be a potentially novel resistant gene-capturing tool kit.