Objective To evaluate the performance of the Sysmex UF1000i automatic urine screening system in the quantitative culture of fast-growth aerobic/facultative bacteria. Methods and Results A standard procedure was used to recover fast-growth aerobic/facultative micro-organisms in 1225 samples, applying (Sysmex (R)) flow cytometry for parallel bacteria and leucocyte counts. According to the area under the receiver operating characteristic curve, the optimal cut-off values to detect bacteriuria >105 colony forming units (CFU) ml-1 were 690/mu l for bacteria and 38/mu l for leucocytes (sensitivity, 92%; specificity, 65%; positive predictive value [PPV], 39%; and negative predictive value [NPV], 97%). The use of a single cut-off point of 150 bacteria mu l-1 to detect significant bacteriuria of >105 CFU ml-1 or of =104 CFU ml-1 plus leucocyturia obtained similar results (sensitivity, 89%; specificity, 54%; PPV, 31%; and NPV, 96%) and allowed 45.7% of the samples to be rapidly excluded. Conclusions The Sysmex UF1000i system can be adapted for bacteriuria screening by the use of an appropriate cut-off point. Significance and Impact of the Study This screening system significantly reduces the workload and produces very few false positives and negatives.