The effect of three methods (mechanical, chemical, and thermal pretreatment) were tested to improve methane production and anaerobic biodegradability of swine wastes. The first experiment was designed to determine the biodegradability enhancement through the separation of liquid and solid matrix by using a 0.25 mm pore size screen (mechanical pretreatment). The second approach was the treatment of swine waste by the addition of a flocculant agent and strong chemicals such as acid (HCl) and alkali (NaOH). The third pretreatment studied was thermal application (170 degrees C provided by vapor). The soluble COD was increased by 57% and 32% during the pretreatment period with alkali and thermal application, respectively. In addition, these two pretreatments gave the highest enhancement on methane production with regard to the untreated sample. Meanwhile, the addition of a flocculant improved the methane production of the liquid fraction but not the solid one. On the other hand, mechanical pretreatment did not show any important enhancement. Biodegradability percentage followed the same trend as methane productivity. (C) 2008 Elsevier Ltd. All rights reserved.