D-Lactic acid and pyruvic acid are two important building block intermediates. Production of D-lactic acid and pyruvic acid from racemic lactic acid by biotransformation is economically interesting. Biocatalyst prepared from 9 g dry cell wt l(-1) of Pseudomonas stutzeri SDM could catalyze 45.00 g l(-1) DL-lactic acid into 25.23 g l(-1) D-lactic acid and 19.70 g l(-1) pyruvic acid in 10 h. Using a simple ion exchange process, D-lactic acid and pyruvic acid were effectively separated from the biotransformation system. Co-production Of D-lactic acid and pyruvic acid by enantioselective oxidation of racemic lactic acid is technically feasible. (C) 2008 Elsevier Ltd. All rights reserved.