A mathematical model is proposed to calculate the enzyme activity in different concentrations of the organic solvent in hydro-organic mixtures. The accuracy and predictability of the model have been evaluated employing experimentally determined xanthine oxidase activity in five hydro-organic mixtures by using absolute percentage mean deviation (APMD). The obtained APMD for correlative and predictive studies were 5.3% and 7.6%, respectively. (C) 2009 Elsevier Ltd. All rights reserved.