Nitrous oxide (N2O) is a highly potent greenhouse gas; however, the characteristics of N2O production during denitrification using poly-beta-hydroxyalkanoates (PHA) as a carbon source are not well understood. In this study, effects of anaerobic reaction time (AnRT) on PHA formation, denitrifying phosphorus removal and N2O production were investigated using a laboratory-scale anaerobic/anoxic/oxic sequencing batch reactor (An/A/O SBR). The results showed that operation of the An/A/O SBR for 0.78 SRT (47 cycles) after the AnRT was shortened from 90 min to 60 min resulted in anaerobically synthesized PHA improving by 1.8 times. This improvement was accompanied by increased phosphorus removal efficiency and denitrification. Accordingly, the N2O-N production was reduced by 6.7 times. Parallel batch experiments were also conducted with AnRTs of 60,90 and 120 min. All results indicated that in addition to the amount of anaerobically synthesized PHA, the kinetics of PHA degradation also regulated denitrifying phosphorus removal and N2O production. (C) 2011 Elsevier Ltd. All rights reserved.