화학공학소재연구정보센터
Bioresource Technology, Vol.102, No.20, 9368-9373, 2011
11 alpha-Hydroxylation of 16 alpha,17-epoxyprogesterone by Rhizopus nigricans in a biphasic ionic liquid aqueous system
11 alpha-Hydroxylation of 16 alpha,17-epoxyprogesterone (EP) by Rhizopus nigricans is an essential step in the synthesis of many steroidal drugs, while low conversion of the biohydroxylation is a tough problem to be solved urgently in industry. Two ionic liquids (ILs) of [BMIm][PF(6)] and [BMIm][NTf(2)] were used in the biotransformation of EP by R nigricans. The results indicated that the conversion carried out in [BMIm][PF(6)]-aqueous biphasic system was greatly increased to above 90% at 18 g/L feeding concentration. A simplified mechanism was proposed to explain the improvement of the bioconversion in a biphasic ionic liquid aqueous system. Besides, successive three batches of bioconversion were carried out in the biphasic system with a total conversion of 87% at phase ratio 10 and 75% at phase ratio 5, respectively. Since recycling of the [BMIm][PF(6)] is quite easy, there is a great potential for the application of ILs in fungi biotransformation to implement green production. (C) 2011 Elsevier Ltd. All rights reserved.