A combination of a twin-screw extrusion and an acid-catalyzed hot water extraction process performed at a bench-scale was used to prepare high monomeric xylose hydrolysate for cellulosic production. The influences of the screw speed (30-150 rpm), barrel temperature (80-160 degrees C) and corresponding specific mechanical energy of the extruder on the structural properties of the pretreated rice straw, sugar concentration and conversion were investigated. The optimal condition for the extrusion step was determined to be 40 rpm with 3% H(2)SO(4) at 120 degrees C; the optimal condition for the extraction step was determined to be 130 degrees C for 20 min. After the pretreatment at the optimal condition, 83.7% of the xylan was converted to monomeric xylose, and the concentration reached levels of 53.7 g/L Finally, after the subsequent enzymatic hydrolysis, an 80% yield of the total saccharification was obtained. (C) 2011 Elsevier Ltd. All rights reserved.