| 초록 |
An aptamer is a single-stranded oligonucleotide that can bind proteins, small-molecule compounds or living cells with high affinity and specificity. In this study, a highly sensitive method to detect Escherichia coli in the solution using antibody conjugated magnetic nanoparticles and E. coli specific RNA aptamer has been developed. Detecting bacteria in a very sensitive and rapid manner is very important in medical and environmental fields. For sensitive detection of E. coli, anti-E. coli antibody conjugated magnetic nanoparticles were mixed with E. coli sample together with RNA aptamers. The nanoparticles, E. coli, and RNA aptamer complex was collected by magnetic bar. The collected E. coli-bound RNA aptamers were released by heating from E. coli and the amount was measured by real-time PCR(RT-PCR). This method allowed an accurate quantification of E. coli using RNA aptamer. |
