| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Single yeast cell traps for monitoring chromosome dynamics |
| 초록 |
Movement of chromosome conducts significant roles in various cellular process such as DNA replication, genetic recombination, epigenetic phenomena during mitosis and meiosis. Here, we present the microfluidic based single yeast cell traps for monitoring the movement of chromosome at meiosis (“prophase I”) stage. The existence of cell in the aperture trap makes the fluidic pressure difference which acts a driving force for sequential cell trapping. After trapping cells, sporulation medium is provided to induce meiosis at a specific time. In conclusion, we demonstrate the tracking and quantification of chromosome dynamics in yeast cells and obtain key measurement that the meiotic chromosome motion requires both actin and telomere/nuclear envelop association. We believe that this microfluidic approach will be useful for investigating other cell biological processes requiring single cell feature tracking and quantification. |
| 저자 |
홍우경1, 진시형1, 이성식2, 김근필3, 이창수1
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| 소속 |
1충남대, 2ETH Zürich, 3중앙대 |
| 키워드 |
single cell monitoring; sing cell trapping
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| E-Mail |
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