| 초록 |
The gene deletion of pflB and ldhA improved 2,3-butanediol production yield, while the deletion of ptsG increased xylose consumption rate significantly in the presence of glucose at previous research. The constructed strain, EMY-22, achieved high production yield with low aeration. However with higher aeration, production yield became lower and productivity became higher, also shorter fermentative time with 2-ketogluconate accumulation. To solve the problem, strain was overexpressed glucose transporter gene (galP) and 2,3-butanediol production operon (budABC). With gene overexpression, strain achieved higher yield with higher aeration condition. Also pyruvate synthesis pathway was overexpressed by pykF and malic enzyme gene overexpression. With pykF and malic enzyme gene overexpression, strain produced 2,3-butanediol with lower 2-ketoglconate accumulation with stable pH condition, adding CaCO3. |
