| 학회 | 한국공업화학회 |
| 학술대회 | 2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 | 23권 2호 |
| 발표분야 | 포스터_생물공학 |
| 제목 | Improved 2,3-butanediol yield and productivity from glucose-xylose mixture by metabolic engineering of Enterobacter aerogenes for reduced byproducts production |
| 초록 | The pathway engineering of Enterobacter aerogenes was attempted to improve its production capability of 2,3–butanediol using glucose and xylose mixture as carbon source. The constructed strain, EMY-22, achieved high production yield with low aeration. However with higher aeration, production yield became lower and productivity became higher, also shorter fermentative time with 2-ketogluconate accumulation. To solve the problem, strain was overexpressed glucose transporter gene (galP) and 2,3-butanediol production operon (budABC). With gene overexpression, strain achieved higher yield with higher aeration condition. Also pyruvate synthesis pathway was overexpressed by pykF and malic enzyme gene overexpression. With pykF and malic enzyme gene overexpression, strain produced 2,3-butanediol with lower 2-ketoglconate accumulation with stable pH condition, adding CaCO3. |
| 저자 | 이보영, 김덕균, 오민규 |
| 소속 | 고려대 |
| 키워드 | Enterobacter aerogenes; metabolic engineering; 2; 3-butanediol |
