| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Optimization of hexanoic acid production in Escherichia coli through fine-tuning of atoB expression |
| 초록 |
Here, we demonstrate that rebalancing of metabolic fluxes at acetyl-CoA can substantially improve the titer and productivity of hexanoic acid in recombinant E. coli. First, the expression of mhact increased hexanoic acid production by 47% compared to that with an E. coli thioesterase, TesB. Next, metabolic flux was optimized at the actyl-CoA branch point by fine-tuning the expression level of the gene for acetyl-CoA acetyltransferase (AtoB). Four different synthetic 5'-UTRs were designed for atoB using UTR Designer. The flux-optimized variant produced 528 mg/L hexanoic acid in 36h, and this titer represents an 8.7-fold increase compared to that of the non-optimized parental strain. Notably, the productivity of the optimized strain was 14.7 mg/L/h and this value was the highest among the recombinant E. coli strains when using a similar inoculum size for fermentation. Our results show that fine-tuning the expression level of atoB is critical for production of hexanoic acid. |
| 저자 |
김성경1, 장성호1, 임재형1, 전병승2, 김정연3, 김경헌3, 상병인2, 정규열1
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| 소속 |
1포항공과대, 2한양대, 3고려대 |
| 키워드 |
hexanoic acid; acetyl-CoA acetyltransferase; UTR Designer
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| E-Mail |
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