| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Signal Amplification of Riboswitch-based Hybrid Inputs by Modular and Titratable Toehold Switches |
| 초록 |
The riboswitches are attractive sensor modules for use in synthetic biology applications. However, the tuning of the riboswitches was constrained by their difficulties and narrow range of modulation. Therefore, novel molecular parts and strategies for straightforward tuning of the fold-change of riboswitch circuits are needed. In this study, we devised a toehold switch-based modulator approach that combines a hybrid input construct consisting of a riboswitch and transcriptional repressor and de-novo-regulator, toehold switches. Optimization strategies that balancing the expression levels of the toehold switch greatly improved the fold-change(887-fold). Further characterizations confirmed low leakiness and high orthogonality of five toehold switch pairs, indicating the broad applicability of this strategy to riboswitch tuning. These results demonstrate that toehold switches can provide programmable and modular plug-and-play genetic parts for the response tuning of riboswitch circuitry. |
| 저자 |
황윤희1, 김성경1, 장성호2, 김종민1, 정규열1
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| 소속 |
1포항공과대, 2인천대 |
| 키워드 |
Synthetic biology; Riboswitch; Toehold Switch
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| E-Mail |
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