| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Tuning of Coenzyme B12 Riboswitch sensor for signal amplification with toehold switch |
| 초록 |
The riboswitches are attractivesensor modules for use in synthetic biology applications. However, the tuningof the riboswitches was constrained by their difficulties and narrow range ofmodulation. Therefore, novel molecular parts and strategies for straightforwardtuning of the fold-change of riboswitch circuits are needed. Here, we devised atoehold switch-based modulator approach that combines a hybrid input constructconsisting of a riboswitch and transcriptional repressor and de-novo-regulator,toehold switches. Optimization strategies that balancing the expression levelsof the toehold switch greatly improved the fold-change(261-fold). Furthercharacterizations confirmed low leakiness and high orthogonality of fivetoehold switch pairs, indicating the broad applicability of this strategy toriboswitch tuning. These results demonstrate that toehold switches can provideprogrammable and modular plug-and-play genetic parts for the response tuning ofriboswitch circuitry. |
| 저자 |
이혜경1, 정규열1, 김종민1, 김성경1, 장성호2
|
| 소속 |
1포항공과대, 2인천대 |
| 키워드 |
Riboswitch; coenzyme B12; Toehold Switch
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| E-Mail |
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