| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터) |
| 권호 |
16권 2호, p.1548 |
| 발표분야 |
생물화공 |
| 제목 |
Enzyme-Catalyzed Resolution of Racemate using Enzyme Functionalized Silica Nanoparticles in the Presence of Surfactants |
| 초록 |
The enzyme-catalyzed resolution of racemic naproxen 2,2,2-trifluoroethyl thioester was performed by lipase immobilized on silica nanoparticles using a covalent bonding method. To increase the conversion and reaction rate of this resolution, the effect of non-ionic surfactants (M-SA 1025 and SN 20) was investigated. The optimal reaction conditions such as temperature and loading amount of immobilized lipase were also determined. The addition of M-SA 1025 resulted in the increase in reaction rate (VS), conversion (XS) and enantioselectivity (E value) comparison with SN 20 and the control. The reaction (VS: 1.913ⅹ10-2 mM/h, XS: 95.5%, E: 131.11) performed in a mixture containing M-SA 1025 at 50 °C with 48 U/mL of immobilized lipase was markedly improved the resolution of racemic naproxen 2,2,2-trifluoroethyl thioester compared to other conditions. |
| 저자 |
송윤석1, 이희욱1, 김성봉1, 장은지1, 이상준1, 박철환2, 김승욱1
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| 소속 |
1고려대, 2광운대 |
| 키워드 |
Lipase; Resolution; Naproxen; Surfactant; Silica nanoparticle; Immobilization
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| E-Mail |
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| 원문파일 |
초록 보기 |
