| 초록 |
Extracellular vesicles (EVs) contain useful biomaterials for disease diagnosis and therapeutic applications. Accordingly, an efficient concentration method is necessary for the large-scale production of EVs from bulk liquid samples for their clinical purpose. However, current EV concentration methods, including ultrafiltration, are limited to efficiency. In this study, we developed the efficient EV concentration method using super absorbent polymer (SAP) beads. SAP beads absorb water, via nano-sized channels but expel and thereby concentrate EVs. Consequently, the beads drastically enrich EVs without affecting EV characteristics and increase the purity of the concentrated EV solution by absorbing protein impurities. To further demonstrate the versatility of the method, we showed that SAP beads successfully enrich EVs in the culture medium. We believe the newly developed method in this study will facilitate the use of EVs for various applications. |
