| 학회 |
한국고분자학회 |
| 학술대회 |
2021년 가을 (10/20 ~ 10/22, 경주컨벤션센터) |
| 권호 |
46권 2호 |
| 발표분야 |
고분자합성 |
| 제목 |
High-throughput screening of Y. Lipolytica cell-laden alginate gel using pneumatic bilayer valve-based microfluidic sorter |
| 초록 |
Biofuels derived from microalgae showed promising potential as a future renewable bioenergy source. Here, we present a microfluidic droplet sorter (μFADS) for screening randomly mutated Yarroiwa Lipolytica library and sort out the target subsets exhibiting higher growth rate and increased lipid production First, cell-encapsulated alginate droplets are generated within the flow-focusing device, followed by alginate gelation through calcium release by lowering pH. The acetic acid in the continuous phase diffused into the droplet due to the difference in solubility, resulting in pH decrease. Then, the Y. Lipolytica cells were cultured in alginate gels that functioned as an individual micro-reactor and re-injected into μFADS to evaluate their capacity and sort target cells. As a result, the alginate gels containing cells showing 1.5 – 2 times higher growth and lipid production rate were selectively retrieved compared to the cells in the waste channel. |
| 저자 |
김재성, 진시형, 이병진, 김진경, 오서연, 이창수
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| 소속 |
충남대 |
| 키워드 |
Microfluidic; Pneumatic bilayer valve; alginate gel; Yarrowia Lipolytica
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| E-Mail |
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