| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Fast antimicrobial susceptibility testing against bacteria using droplet-based microfluidic device |
| 초록 |
We present a microfluidic device for antimicrobialsusceptibility testing(AST) against bacteria in a single device. Not onlyintegrating several flow-focusing channels but also controlling the flowresistance of two aqueous phases, we could produce the cell-encapsulateddroplets having linear concentration gradient of antibiotics. Using thisstrategy, we tested gentamicin against E.coliO157:H7 to determine the minimum inhibitory concentration(MIC). Growth indroplets were measured by counting the number of cells through fluorescencemicroscopy. E.coli O157:H7 whichproduces green fluorescence protein(GFP) facilitated cell counting. Screeningthe growth profile of cells in droplets having different concentration ofgentamicin resulted in MIC determination in only 4-6 h using our device. Thisresults show that our platform has the great potential to be used for AST withother pairs of bacteria (i.e. gram-positive bacteria) and antibiotics |
| 저자 |
김진경1, 김재성1, 이병진1, 정헌호2, 이창수1
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| 소속 |
1충남대, 2전남대 |
| 키워드 |
Microfluidics; Parallel Droplet Generation; Concentration gradient; Gram-negative bacteria; Antibiotics
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| E-Mail |
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