| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Preparation of microfluidicsorter for screening of Yarrowia Lipoltica producing high lipid accumulation |
| 초록 |
Asbiofuels, they are a perfect substitute to liquid fossil fuels with respect tocost, renewability, and environmental concerns. Biofuels derived frommicroalgae showed promising potential as a future renewable bioenergy source. Here,we demonstrate a screening randomly mutated Yarroiwa Lipolytica library and sort outthe target subsets exhibiting higher growthrate and increased lipid production by using valve based microfluidic sorter (μFADS). First,we generate the cell-encapsulated alginate droplets through a flow-focusing deviceand perform the gelation process through calcium ion releasing by lowering pHcondition. Then, the Y. Lipolyticacells were cultured in alginate gels that functioned as an individualmicro-reactor and re-injected into μFADS to evaluate their capacity and sort target cells. As a result, the alginate gelscontaining cells showing 1.5 – 2 times higher growth and lipid production ratewere selectively retrieved compared to the cells in the waste channel. |
| 저자 |
윤혜원, 김재성, 진시형, 이병진, 김진경, 이창수
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| 소속 |
충남대 |
| 키워드 |
microfluidics; Yarrowia Lipoltica; sorter
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| E-Mail |
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